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Figure 3 | Molecular Brain

Figure 3

From: ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate transmembrane cargos in hESC-derived human neurons

Figure 3

hSnf7-1 and hSnf7-2 are expressed in human post-mitotic neurons. (A) qRT-PCR showed that hSnf7-2 was highly expressed in human neurons, while hSnf7-3 was barely detectable. (B) hESCs were immunostained with hSnf7-2. hSnf7-2 was mostly localized to cytoplasm. (C) Nucleus staining (indicated by DAPI staining) of hESCs as shown in panel B. (D) Immunostaining showed that hSnf7-1 was mostly expressed in the cytoplasm of human neurons. Bar: 20 mm. (E) Immunostaining with a hSnf7-2-specific antibody reveals hSnf7-2 in both the nucleus and cytoplasm of human neurons. Bar: 20 μm.

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Molecular Brain

ISSN: 1756-6606