Figure 1
From: Wnt proteins regulate acetylcholine receptor clustering in muscle cells
Wnt proteins induce AChR clusters in muscle cells. A, C2 myotubes were stimulated with conditioned media containing Wnt proteins or control medium for 16 h. AChR clusters were visualized by R-BTX staining and indicated by arrows. B. Quantification data of A. AChR clusters greater than 4 μm in length were quantified. *, p < 0.01, Student's t test. C, Wnt mRNAs are expressed in muscle. Total RNAs were extracted from skeletal muscles of P0, P15, P30 and adult mice and used for qRT-PCR. Relative expression was shown in histograms.