Figure 4

Post-training infusion of anisomycin into the striatum impaired long-term fear memory. (A) Experimental design. Mice were injected with 0.5 μl of ACSF or anisomycin into the striatum immediately after auditory fear conditioning, and tested 24 h later. (B) Freezing responses of ACSF (n = 8) and anisomycin (n = 7) groups on the conditioning at 0.3 mA (left) and test (right) days. Solid lines and a thin arrow represent tone and footshock, respectively. (C), Mice were injected with ACSF or ANI immediately after the conditioning or 1 h or 3 h after the conditioning (0.3 mA), and tested 24 h later. Bar graph shows the average freezing responses during tone presentation (3 min) of ACSF group with ACSF injections right after conditioning (ACSF 0 h; n = 8 as in Figure 5B), ANI groups with ANI injections immediately (ANI 0 h; n = 7 as in Figure 5B) or 1 h (ANI 1 h; n = 9) or 3 h (ANI 3 h; n = 6) after conditioning. The same data set as the ACSF group and ANI groups in (B) are also shown. *, P < 0.05; **, P < 0.01. (D), Mice were injected with ACSF or ANI into the striatum immediately after auditory fear conditioning with a high-intensity footshock (1.0 mA), and tested 24 h later. Freezing responses of ACSF (n = 7) and ANI (n = 7) groups on the conditioning (left) and test (right) days. Solid lines and a thick arrow represent tone and footshock (1.0 mA), respectively. ANI, anisomycin; BLA, basolateral amygdala; CeA, central amygdala; CP, caudate putamen.