Figure 2

The capability of firing spikes is higher during AISS expression and induction than the evoked spikes. A ~ C) Input–output curves (depolarization intensities versus spike frequencies) are measured under the conditions of the evoked spikes and subsequent AISS expression in GABAergic neurons. A) shows the spikes evoked by a depolarization pulse (100 pA/1 second) at a neuron. B) shows the spikes evoked by this depolarization pulse during AISS expression at this neuron. C) illustrates spikes per second versus stimulus currents under the conditions of control (blue symbols) and AISS expression (red symbols, n = 8). D ~ E) Instantaneous spike frequency (1/inter-spike interval) are measured in the spike trains of inducing AISS from the first train and the train just before AISS onset in GABAergic neurons. D) shows the spikes evoked by a depolarization pulse (150 pA/3 second) in the first train (blue trace) and the train just before AISS onset (green trace), as well as AISS expression (gray trace) at a neuron. Calibration bars are 10 mV and 4 seconds. E) shows instantaneous spike frequency versus time for the spikes induced in the first train (blue symbols) and in the train just before AISS onset (green symbols) at this cell. F) Statistical analysis shows instantaneous spike frequency versus time for the spikes induced in the first train (blue symbols) and in the train just before AISS onset (green symbols, asterisks, p < 0.01, n = 29 neurons).