Fig. 6

Neural migration of upper-layer neurons is impaired by loss of Hif1α. Apoptotic cells and BrdU-incorporated neurons in coronal sections of WT and KO cortices at E18.5 are detected with anti-cleaved caspase3 and anti-BrdU antibodies (A–D). Scale bars, 100 μm (A–D). Three independent experiments are performed and one representative image is shown, respectively. The percentage of BrdU+ cells in the cortex (bins 1–5, 500 μm-wide) is presented as a histogram (G). The total number of BrdU+ cells in the cortex (500 μm-wide region) is presented as a bar graph (H). Gray bar, WT; black bar, KO. Data are the mean ± S.E.M of four sections. Statistical differences were assessed using Student’s t-test, * p < 0.05. WT, wild type; KO, knockout