Fig. 1

Olfr78 was expressed in AVP neurons in the hypothalamus. a–d, Olfr78 immunoreactivity (Olfr78-IR) was detected in the a paraventricular hypothalamus (PV), b supraoptic nucleus (SON) and c internal layer (IL) of the median eminence (ME) of the hypothalamus and in the d choroid plexus (ChP) by using an Alexa Fluor 488-conjugated anti-rabbit IgG secondary antibody (Olfr78-Rb488). e, f Olfr78-IR (green; Olfr78-Rb488) colocalization with arginine vasopressin (AVP)-IR in the e PV and f SON was detected by using an Alexa Fluor 594-conjugated anti-guinea pig IgG secondary antibody (red; AVP-GP594). g, h Olfr78-IR (green) colocalization with oxytocin (OXT)-IR (red) in the g PV and h SON was detected directly by using a DyLight 594-conjugated anti-OXT primary antibody (red; OXT-594conj). i Quantification of neurons expressing solely Olfr78, AVP or OXT or coexpressing Olfr78/AVP or Olfr78/OXT. n = 70 (PV) or 66 (SON) cells from 3 mice for AVR/Olfr78 and 72 (PV) or 58 (SON) cells from 3 mice for OXT/Olfr78. See Figure S4c for the means and standard deviations. j–m Olfr78-IR (green) was distinct from j CD31-IR in the endothelium and colocalized k with Iba1-IR in microglia/macrophages (red); l with TNFα-IR in M1 macrophages (red); and m with MMR-IR in M2 macrophages (red). Nuclei are coloured blue in (e–h and j–m). 3V third ventricle; opt, optic tract, EL external layer, Arc arcuate hypothalamic nucleus, Hip hippocampus, MHb medial habenular nucleus, D3V dorsal third ventricle, TNFα tumour necrosis factor α, MMR macrophage mannose receptor