Fig. 3

The HBVSMC secretome expands the time window of neurite initiation and protects against neuronal death. a Images of hippocampal neurons that were initiating nascent neurites. White and black arrows indicate neurite initiations that occurred by 24 h after plating. Red arrows point to neurite initiations that were generated 24–40 h after plating. b Quantification of the percentage of neurons that possessed the ability to generate nascent neurites. c Cumulative neurite numbers across the entire imaging period. P values in black and gray refer to statistical comparisons between two consecutive time points in the same group, while P values in magenta refer to statistically significant differences between two groups at the same time point. d Quantitative analysis of neuron density over time. e Representative images of neurons treated with or without HBVSMC-CM that immunostained with an anti-Tuj1 antibody. f Quantification of Tuj1⁺ cell density in e. g Representative images of neurons treated with or without MBVSMC-CM that immunostained with an anti-Tuj1 antibody. h Quantification of Tuj1⁺ cell density in g. i Fold change analyses of Tuj1⁺ cell density in HBVSMC-CM and MBVSMC-CM compared with that in control group