Fig. 1

Specific ACSA-2-immunostaining in the gray matter (GM) of spinal cord in adult mice. a Immunolabeling with an ACSA-2 antibody in the fourth lumbar (L4) spinal cord of Aldh1l1-EGFP mice. The dashed line indicates the boundary between GM and white matter (WM). b Representative immunohistochemical images of ACSA-2 and matched IgG control antibodies in the spinal dorsal horn (SDH) of Aldh1l1-EGFP mice. The immunofluorescence intensities of ACSA-2 and IgG was enhanced to a saturated level in the GM by increasing the gain of image acquisition. Scale bars, 500 μm (a) and 100 μm (b)