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Fig. 4 | Molecular Brain

Fig. 4

From: A method for selective and efficient isolation of gray matter astrocytes from the spinal cord of adult mice

Fig. 4

Optimization of tissue processing for enhancing the yield of spinal GM astrocytes.  a, b Effects of myelin removal (a) and enzymatic reaction (b) on the yield of isolated ACSA-2high cells from the hemisection of the L3/4 spinal cord (n = 5 mice). c Representative FACS plots of cells stained with the ACSA-2 antibody under the optimized myelin removal and tissue dissection protocol. d qPCR analysis of cell type-specific markers in FACS-sorted ACSA-2high cells (n = 5 mice). Data show mean ± SEM

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Molecular Brain

ISSN: 1756-6606