Fig. 4

CD11c⁺ microglia in the prenatal mouse brain. (A) Representative immunofluorescence images of Venus⁺ (CD11c⁺; green) cells in the whole brain of Itgax-Venus mice at E14.5. The parenchymal brain region was indicated by a dashed line. Venus⁺ cells (green) were colocalized with IBA1 (red). Scale bars, 1000 μm (left panel) and 100 μm (right panel). (B) Flow cytometry analysis of the percentage of CD11c⁺ microglia per total microglia (CD11b⁺ CD45^low CD206^neg cells) at E12.5, E14.5, and E16.5 (n = 6–8 mice for each time point tested). Data are shown as means ± SEM. **P < 0.01, ***P < 0.001, and ****P < 0.0001