Fig. 5

Temporal analysis of CD11c⁺ microglia in the mouse spinal cord. (A) Representative immunofluorescence images of Venus⁺ (CD11c⁺; green) cells in the spinal cord of Itgax-Venus mice at E12.5 and E14.5. Venus⁺ cells (green) were colocalized with IBA1 (red) in the spinal cord. Scale bars, 500 μm. (B) Representative scattered plot of CD11c^neg and CD11c⁺ microglia (blue and red square, respectively) in the spinal cord of Itgax-Venus mice at P4. (C) qPCR analysis of P2ry12, Igf1, Clec7a, and Trem2 mRNA in FACS-isolated CD11c^neg and CD11c⁺ microglia in the spinal cord at P4 (n = 7 mice). Values represent the relative ratio of the mRNA levels (normalized to Actb mRNA) of CD11c^neg microglia group. (D) Temporal analysis of the percentage of CD11c⁺ and CD11c^high microglia per total microglia (CD11b⁺ CD45^low CD206^neg cells) during development and adult (n = 4–10 mice for each time point tested). Data are shown as means ± SEM. ***P < 0.001, and ****P < 0.0001