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Fig. 1 | Molecular Brain

Fig. 1

From: A novel mouse model for investigating α-synuclein aggregates in oligodendrocytes: implications for the glial cytoplasmic inclusions in multiple system atrophy

Fig. 1

The expression of human αsynGFP in CNP-SNCAGFP transgenic (Tg) mice. A The transgenic construct. B Western blot images of whole-brain SDS-soluble fraction with anti-αsyn and GFP antibodies in both genotypes (2 months old). The expression of endogenous αsyn (arrow) and transgenic human αsynGFP fusion protein (arrowhead) is shown. C Fluorescence microscopy showed the αsynGFP expression in in both genotypes (2 months old). Scale bar = 200 µm. D Immunofluorescent staining of various cellular makers with αsynGFP in the striatum of Tg mice (2 months old). The merged images include DAPI (blue). GFP signals were observed exclusively in OLGs. Note that anti-CNP antibodies strongly labeled the plasma membrane in OLGs. Scale bar = 10 µm. Wt, wild-type; αsyn, alpha-synuclein; Ctx, cortex; CC, corpus callosum; Str, striatum

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Molecular Brain

ISSN: 1756-6606