Fig. 3

αsynGFP aggregates in OLGs developed in a centripetal manner in CNP-SNCAGFP Tg mice inoculated with αsyn PFFs. A Fluorescence micrographs of the corpus callosum of Tg mice inoculated with αsyn PFFs. Small αsynGFP aggregates (GFP dots) were observed as early as 1 mpi (arrows), and their size and signal intensity increased over time. Scale bar = 50 µm. B Quantification of the GFP dot area in (A) at 1, 4, and 12 mpi (n = 6). C Fluorescence micrographs of GFP and immunostaining with anti-TPPP antibody in the corpus callosum of Tg mice at 1, 4, and 12 mpi. The merged images include DAPI (blue). GFP dots were initially observed in the OLG process and developed toward the cell body. Scale bar = 10 µm. D Quantification of the number of aggregates in TPPP-positive cell bodies in (C) at 1, 4, and 12 mpi (n = 6 at each time point). E The development of GFP dots is illustrated. Tukey’s multiple comparisons test was performed in (B) and (D); *p < 0.05, **p < 0.01, ****p < 0.0001, n.s., not significant. Data indicate the mean ± SEM. mpi, months post-inoculation; CC, corpus callosum; Str, striatum