Fig. 4

Thread-like αsyn aggregates in the process of OLGs in MSA brains. A Immunohistochemical staining of human autopsy brains with anti-pαsyn antibody. Some of the pαsyn-positive aggregates in MSA brains showed thread-like structures continuous with the cytoplasmic inclusions. The following regions were analyzed: the frontal lobes (C, D, M1-3), the putamen (M4), and the cerebellum (M5). Scale bar = 50 µm. B Double immunofluorescent staining of thread-like aggregates with anti-pαsyn antibody and various cellular markers in DLB and MSA brains. The merged images include DAPI (blue). Lewy neurites in DLB brains and some of the thread-like pαsyn-positive aggregates in MSA brains co-localized with MAP2 and NF (arrows). Other pαsyn-positive aggregates in MSA brains were not co-localized with neuronal makers but were co-localized with CNP and were continuous with GCIs (arrowheads). Note that anti-CNP antibodies strongly labeled the plasma membrane in OLGs. Scale bar = 10 µm. C, control; D, DLB (dementia with Lewy bodies); M, MSA. NF, neurofilament; pαsyn, phosphorylated α-synuclein