Fig. 5

Inoculation of brain homogenates from MSA samples induced distinct αsyn aggregates in OLGs in CNP-SNCAGFP Tg mice. A Fluorescence micrographs of GFP and immunostaining with anti-pαsyn (red) and anti-NeuN (gray) antibodies in the striatum of Tg mice treated with BH from DLB or MSA samples at 2 mpi and 6 mpi. The merged images include DAPI (blue). GFP dots (arrowheads) represent aggregates in OLGs. Aggregates that were immunopositive for pαsyn and not co-localized with GFP were neuronal (arrows). B Comparison of the number of αsyn aggregates in neurons and OLGs in the striatum (bregma + 0 mm) of Tg mice treated with BH from DLB or MSA. A single data point represents the average number of αsyn aggregates of four mice at 2 mpi and two mice at 6 mpi, respectively. Neither the neuronal aggregate nor GFP dot exhibited a significant increase over time in the DLB BH-treated group. In contrast, the MSA BH-treated group displayed a notable increase only in GFP dots at 6 mpi. Scale bar = 20 µm. Student’s t-test was performed in (B); *p < 0.05, n.s., not significant. Data indicate the mean ± SEM. BH, brain homogenates; D, DLB (dementia with Lewy bodies); M, MSA; mpi, months post-inoculation; pαsyn, phosphorylated α-synuclein