Fig. 1
From: TDP43 interacts with MLH1 and MSH6 proteins in a DNA damage-inducible manner
TDP43 Interacts with MMR factors in a DNA Damage-Inducible Manner. A Representative immunofluorescence (IF) images and quantitation of proximity ligation assay (PLA) in neuronal cell cultures show a significant increase in the number of foci per nucleus for TDP43-MLH1 interaction following methylmethanesulfonate (MMS) treatment. This interaction is abolished by TDP43 knockdown. B Representative IF images and quantitation of PLA in neuronal cell cultures show a significant increase in the number of foci per nucleus for TDP43-MSH6 interaction following MMS treatment which is also abolished by TDP43 knockdown. C Western blot analysis of co-immunoprecipitation assay. Pulldown of TDP43 and its interacting proteins show detection of both MLH1 and MSH6. D Schematic showing the ventral horn region of the human lumbar spinal cord used to measure TDP43-MMR protein interaction. E Representative IF images of PLA showing increased TDP43-MLH1 and TDP43-MSH6 interaction within the ventral horn of the lumbar spinal cord of ALS-affected tissue compared to age-matched controls. F, G Quantitation of PLA immunoreactivity. Scale bars, 5 µm. p