hCav2.1 | Activation V50 (mV) | Activation k | N | Inactivation V50 (mV) | Inactivation k | N |
|---|
wt ΔSSTR | −11.80 ± 0.46 | 3.65 ± 0.08 | 13 | −45.06 ± 0.73 | 6.42 ± 0.12 | 12 |
S218L ΔSSTR | −16.78 ± 031*** | 5.21 ± 0.16*** | 16 | −60.47 ± 0.37*** | 5.28 ± 0.18** | 8 |
wt +SSTR | −15.44 ± 0.30### | 3.99 ± 0.06 | 17 | −47.16 ± 0.64 | 6.08 ± 0.18 | 8 |
S218L +SSTR | −22.05 ± 0.44*** | 5.70 ± 0.04*** | 12 | −62.90 ± 0.43*** | 5.24 ± 0.31* | 8 |
- Summary table showing mean values ± SEM of activation and inactivation voltage dependence parameters. N = sample size. P values listed below correspond to results from one-way ANOVA with post hoc Tukey test
- ***: P value < .0001 indicate statistical significance of S218L mutant voltage dependence values as compared to the corresponding wild type
- ###: P value < .0001 from the statistical comparison between activation V50 of wild type channels
- **: P = 0.0009 from statistical comparison between S218L ΔSSTR and wt ΔSSTR
- The significance level obtained by comparing activation V50 and k values between S218L mutant channels was P < .0001, and P = 0.0108, respectively. The significance level obtained for k values between wild type channels was P = 0.0980
- *: P value = 0.0364 from statistical comparison between S218L +SSTR and corresponding wildtype +SSTR
- Comparison between the two wild-type isoforms (V50 P = 0.0821; k P = 0.5809) indicates no statistical difference of inactivation parameters. Comparison between the two mutant channels shows no significant difference in V50 (P = 0.0616), or k (P = 0.9993
