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Fig. 1 | Molecular Brain

Fig. 1

From: Knockout of AMPA receptor binding protein Neuron-specific gene 2 (NSG2) enhances associative learning and cognitive flexibility

Fig. 1

Validation of brain-wide NSG2 knockout in null animals. (A) Western blots of wt C57Bl/6n animals (lanes 1–5) and NSG2 KO animals (lanes 6–10) used for behavioral studies demonstrates complete lack of NSG2 protein in KO animals. (B) Bar graphs of WB band intensity normalized to GAPDH revealed that both wt and KO animals demonstrate normal expression of NSG1 and NSG3. (C-D) Immunohistochemical staining of NSG1 (green), NSG2 (red), and DAPI (blue) on parasagittal brain sections from a wt (C) and KO animal (D). Note specific loss of NSG2 staining in KO section (D). Specificity of NSG1 vs. NSG2 staining is confirmed by robust cerebellar staining of NSG1 antibody in wt brain section (C, lower right panel) while NSG2 signal is relatively low in cerebellum (C, upper right panel)

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Molecular Brain

ISSN: 1756-6606