Fig. 1

Differential expression analysis of Nek6 protein in CIRI model. The mice were divided into sham group and MCAO group. A Neurological deficit scores were evaluated in each group. N = 7. Difference was calculated using Mann Whitney test. **P < 0.01 vs. sham group. B TTC staining was used to detect the degree of cerebral infarct. N = 1. C The expressions of Nek6 in peri-infarct region were analyzed by qPCR and western blot. N = 6. Difference was calculated using unpaired two tailed T test. **P < 0.01 vs. sham group. D–F SH-SY5Y cells were grouped into control and OGD/R. D Cell apoptosis was detected by flow cytometry. E Cell activity was detected by MTT assay. F The levels of Nek6 mRNA and protein were detected by qPCR and western blot. Difference was calculated using unpaired two tailed T test. **P < 0.01 vs. control group