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Fig. 3 | Molecular Brain

Fig. 3

From: Nek6 regulates autophagy through the mTOR signaling pathway to alleviate cerebral ischemia–reperfusion injury

Fig. 3

Nek6 inhibits autophagy through the Akt/mTOR pathway. A SH-SY5Y cells were grouped into OGD/R + pcDNA, OGD/R + pcDNA-Nek6, OGD/R + si-control, OGD/R + si-Nek6. Western blot was used to detect the expressions of p-Akt, Akt, p-mTOR, mTOR, ULK1 and p-ULK1. B, C SH-SY5Y cells were grouped into OGD/R + pcDNA, OGD/R + pcDNA-Nek6, OGD/R + pcDNA-Nek6 + LY and OGD/R + pcDNA-Nek6 + Rapa. B Western blot was used to detect the expressions of LC3 I/II, Beclin 1, p-Akt, Akt, p-mTOR, mTOR, ULK1 and p-ULK1. C Cell apoptosis was detected by flow cytometry. Difference was calculated using one way anova with tukey’s post-hoc. **P < 0.01 vs. OGD/R + pcDNA or OGD/R + pcDNA-Nek6

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Molecular Brain

ISSN: 1756-6606