Fig. 5

Nek6 mRNA was post-transcriptional regulated through m6A modification. A The expressions of Nek6 introns in peri-infarct region of mice (N = 6) or SH-SY5Y cells were analyzed by qPCR. B ActD treatment was used to evaluate Nek6 mRNA stability. C The m6A binding site of Nek6 mRNA was predicted by SRAMP. D MeRIP and qPCR were used to detect Nek6 methylation level in vivo (N = 6) and in vitro. *P < 0.05, **P < 0.01 vs. sham group or control group. n.s. means no significance. E SH-SY5Y cells were grouped into OGD/R + si-control, OGD/R + si-METTL3. MeRIP and qPCR were used to detect Nek6 methylation level. F QPCR and Western blot were used to detect the expressions of Nek6. G ActD treatment was used to evaluate Nek6 mRNA stability. A, B, D–G Difference was calculated using unpaired two tailed T test. *P < 0.05, **P < 0.01 vs. OGD/R