Fig. 1

Experimental pipeline schematic presentation. a Freely behaving mouse with an attached miniscope v3. b Fixed sagittal brain slice with hole above the hippocampus after surgery, 4× magnification (in the right corner fluorescent image of the GCaMP6f fluorescence, 10× magnification). By dotted line GRIN lens boarders are drawn. c CA1 hippocampal neurons activity recorded by the miniscope. Illustration represents sum of 1000 frames from a single recording. d Timescale for neuronal activity visualization by miniscope in the Open-field behavioral test with acute stress modeling as a great external stimulus. e Extracted single neuron activity as active and inactive state using spike method with values “warm” of 50 and “cold” of 0 in “NeuroActivityToolkit”. f Recorded neuronal ensembles activity representation in a binarized form for a single recording (mouse 1, baseline day 1, 182 neurons). White lines indicate single neuron activation