Fig. 2

Shift in the activity properties at the single neuronal level as well as in the entire neuronal ensembles after acute stress modeling. a–c Calcium events number per minute for individual mouse for single-cell activity comparison. d Mean value of calcium events as total neuronal circuits characteristic for all states (baseline (n = 15) vs stress (n = 3), p = 0.0002; baseline (n = 15) vs 3 h (n = 3), p = 0.0009; baseline (n = 15) vs 10 days (n = 3), p = 0.9690; stress (n = 3) vs 3 h (n = 3), p = 0.9467; stress (n = 3) vs 10 days (n = 3), p = 0.0066; 3 h (n = 3) vs 10 days (n = 3), p = 0.0212; Ordinary One-way ANOVA with Tukey test for multiple comparison, F(3,20) = 14.29). e Individual response from the shared neurons between sessions for all states. All comparisons are significantly different with p < 0.0001, except: stress (n = 73 neurons) vs 3 h (n = 64 neurons), p > 0.9999 and baseline (73 neurons) vs 10 days (64 neurons), p > 0.9999. f Percentage of neurons that responded to stress or not for stress, 3 h and 10 days states. (g) Left: representative calcium traces of the same stress activated neuron on day 5 (baseline), stress, 3 h and 10 days states. Middle: representative calcium traces of the same stress inhibited neuron on day 5 (baseline), stress, 3 h and 10 days states Right: representative calcium traces of the same stress non-responsive neuron on day 5 (baseline), stress, 3 h and 10 days states. Scale bars corresponds to 25 s. Kruskal–Wallis test with Dunn’s test for multiple comparisons was used for comparisons in a–e, ns: no significant difference, #: p < 0.05; ##: p < 0.01; ###: p < 0.001; ####: p < 0.0001. Ordinary one-way ANOVA following Tukey post-hoc test was implemented for comparison in d, *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001. All the data presented as mean ± SEM