Fig. 2
From: Distribution and functional significance of KLF15 in mouse cerebellum
KLF15 knockdown virus can effectively knock down mouse cerebellar KLF15. A The positions of five interfering sites on the KLF15 gene. B Real time fluorescence quantitative PCR detection of plasmid knockdown efficiency at different target sites. C Sh-KLF15 virus stereotactic injection into the cerebellum. Target 850 site plasmid as the optimal knockdown target for virus packaging and cerebellum stereotactic injection. D Western blot verifies virus knockdown efficiency on the 21st day after injection. Data are shown as the mean ± SD. **P < 0.01, ***P < 0.001, vs. control group (two-tailed Student’s t-test)