Fig. 4

Loss and morphological abnormalities of Purkinje cells in the cerebellum of KLF15 knockdown mice. A Weighting the cerebellum of control mice and KLF15 knockdown mice. The cerebellum weight of the knockdown group was significantly lower than that of the control group. B Immunofluorescence staining of cerebellum in the control mice and sh-KLF15 mice. KLF15 knockdown mice have significantly thinner granular layer than control group mice. Scale bar = 200 μm. The white line represents the thickness of the granular layer in the control group. C Immunofluorescence staining of cerebellum in the control mice and sh-KLF15 mice. On the right is the quantitative statistics of Purkinje cell number. Scale bar = 200 μm. D Western blot and grayscale analysis of calbindin in the cerebellum of control mice and KLF15 knockdown mice. E Representative images of Golgi staining in the cerebellum of control mice and KLF15 knockdown mice. Scale bar = 80 μm. Sholl analysis images of Purkinje cells (n = 6). Data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, vs. control group. Two-tailed Student’s t-test A, B, C, D and two-way ANOVA followed by Tukey’s test E were used for data comparison