Fig. 3

SB inhibited microglial activation and regulated the microglial polarization after CA. A Representative IHC staining images of Iba-1 in the cortex. B The statistical analysis of Iba-1-positive cell areas in the differently treated mice. C Representative western blotting patterns of Iba-1. D Iba-1 analysis in the cortex at 24 h post-CA/CPR. E Representative images of Iba-1 and CD86 double immunofluorescent staining in the cortex. F Quantification of CD86 cells/ microglial Iba-1 cells positive area. G Representative images of Iba-1 and CD206 double IF staining in the cortex. H Quantification of CD206 cells/microglial Iba-1 cells positive area. Scale bar: 50 µm (200 ×). Data are depicted as mean ± SEM (n = 3/group). ns, no significant, ***p < 0.001, **p < 0.01, *p < 0.05