Fig. 8

SB mediated the activation of microglia M1/M2 polarization and attenuated microglial inflammation. A Representative images of CD86 and Iba-1 double immunostaining. B Quantitative analysis of fluorescence intensity in control or OGD/R BV2 cells with or without SB treatment. C Representative images of CD86 and Iba-1 double immunostaining and D quantitative analysis of fluorescence intensity in control or OGD/R BV2 cells; treated or not-treated with SB. E–G The percentage of CD86 and CD206 positive BV2 cells detected by PE/APC staining with flow cytometric analysis. H-I TNF-α, IL-6, TGF-β, and IL-10 levels in supernatants were assessed by ELISA. Scale bar: 50 µm (200 ×). Data were displayed as mean ± SEM (n = 3/group). ns, no significant, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05