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Table 2 The original grayscale values of Western Blot bands and the corresponding relative expression levels

From: Sodium butyrate attenuates microglia-mediated neuroinflammation by modulating the TLR4/MyD88/NF-κB pathway and microbiome-gut-brain axis in cardiac arrest mice

TLR4-1

GAPDH-1

TLR4-2

GAPDH-2

TLR4-1/GAPDH-1

TLR4-2/GAPDH-2

 

Sham+Vehicle

Sham+SB

CA/CPR+Vehicle

CA/CPR+SB

    

Animals

8699.326

26790.33

9619.598

11497.36

0.324719

0.836679

  

0.324719

0.590954

1.117632

0.92305

     

10811.74

23899.33

8827.719

10304.36

0.452387

0.856698

  

0.452387

0.663425

1.050995

0.804422

     

14216.15

26619.62

5582.77

11344.6

0.534048

0.492108

  

0.534048

0.611695

1.205068

0.827484

     

14480.03

24502.79

4703.062

11392.06

0.590954

0.412837

  

0.836679

0.412837

0.513492

0.699868

     

15838.15

23873.33

3878.477

13264.65

0.663425

0.292392

  

0.856698

0.292392

0.922604

0.520771

     

15791.03

25815.21

4199.648

13771.6

0.611695

0.30495

  

0.492108

0.30495

1.149012

0.608289

     

27629.4

24721.38

7199.184

14020.06

1.117632

0.513492

 

mean

0.582773

        

28684.33

27292.55

11924.01

12924.31

1.050995

0.922604

 

Relative TLR4/GAPDH

0.557196

1.014039

1.917782

1.583892

     

28690.57

23808.26

13911.31

12107.18

1.205068

1.149012

  

0.776266

1.138393

1.803437

1.380334

     

21882.28

23706.5

8049.719

11501.77

0.92305

0.699868

  

0.916391

1.049628

2.067817

1.419908

     

19676.98

24461.03

6267.426

12034.89

0.804422

0.520771

  

1.435686

0.7084

0.881117

1.200927

     

19356.62

23392.13

8138.77

13379.77

0.827484

0.608289

  

1.470037

0.501725

1.583127

0.893609

     
        

0.844425

0.523274

1.971629

1.043784

     

TLR4-1

GAPDH-1

TLR4-2

GAPDH-2

TLR4-3

GAPDH-3

TLR4-1/GAPDH-1

TLR4-2/GAPDH-2

TLR4-3/GAPDH-3

mean

   

Control

Control+SB

OGD/R

OGD/R + SB

Cells

27976.41

33896.85

31099.95

35836.87

19946.22

32070.29

0.825339

0.86782

0.621953

0.771704

1.069502

1.12455

0.805948

1.069502

1.102998

1.493291

1.059064

29697.41

34889.36

29027.66

33881.51

29301

35546.65

0.851188

0.85674

0.824297

 

1.102998

1.110193

1.068152

1.12455

1.110193

1.547392

1.010152

41079.39

35647.46

40391.95

33825.46

44353.84

34496.8

1.152379

1.194129

1.285738

 

1.493291

1.547392

1.666102

0.805948

1.068152

1.666102

1.370118

33279.17

40719.22

24264.34

31126.54

36128.58

34169.77

0.817284

0.779539

1.057326

 

1.059064

1.010152

1.370118