Fig. 3

Differential expression of IEGs in the hippocampus of Trpm2^−/−mice after contextual fear conditioning and fear extinction. (a) The hippocampus was dissected from mice 1 h after CFC, 1 h after E7, or from mice removed from their home cage (HC). (b) mRNA levels of Npas4, c-Fos, Arc and Egr1 in mice that underwent CFC (detailed statistics in Supplementary Information). (c) Npas4, c-Fos and Egr1 mRNA increased in the hippocampus after extinction in WT mice but not in Trpm2^−/− mice. Trpm2^−/− mice contained less Npas4, c-Fos, Arc and Egr1 mRNA than WT mice after FE (day 8). (detailed statistics in Supplementary Information). Relative levels of IEG mRNAs measured in the hippocampus 1 h after contextual fear conditioning. Results for gene of interest mRNAs were normalized with the level of β-Actin. The mRNA levels during CFC and FE are shown as fold changes relative to the levels in the home cage WT. Values represent mean ± SEM (n = 3 to 6 per group). * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent mean ± SEM