Fig. 1

Structural changes in veins were associated with ischemic injury in the acute phase. (A) Sequential live imaging revealed that the terminals of vein branches were enlarged in the peri-infarct region in the acute phase. Arrows, enlarged terminals. The arteries and veins selected in A were pseudocoloured. Blue and v., veins. Red and a., arteries. (B) Statistical charts showing an increase in the size of the terminal in the acute phase. n = 5, before vs. 24 h, p = 0.026; before vs. 72 h, p = 0.001. (C) LSCI under a thinned-skull window was used to detect cerebral blood flow (CBF) changes in the same mice as shown in graph A. (D), (E) and (F) Ligation of the right jugular vein at 24 h (24 h-LJV) decreased the ipsilateral CBF (n = 5 ~ 6, p = 0.0013) and increased the infarct volume 3 days later (n = 6, p = 0.0433). (G) TTC staining showing the infarct size on day 3 postischemia