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Fig. 1 | Molecular Brain

Fig. 1

From: Analysis of neurexin-neuroligin complexes supports an isoform-specific role for beta-neurexin-1 dysfunction in a mouse model of autism

Fig. 1

Generation of epitope-tagged alpha- and beta-Nrxn1 isoforms. (A) Comparison of the expression of Flag-tagged alpha- and beta-Nrxn1 with that of Flag-untagged versions. Schematic drawings showing the insertion site of a Flag-sequence in the cytoplasmic tail of alpha- and beta-Nrxn1 proteins. All the constructs contained an additional HA tag at the N-terminal region. Western blots of HEK293T cells transfected with Flag-tagged and untagged alpha and beta-Nrxn1 (-AS4) constructs were probed with Flag and HA antibodies, as indicated. (B) Expression of alpha- and beta-Nrxn1 splice isoforms. Drawings showing the generation of Flag-tagged alpha- and beta-Nrxn1 constructs containing (AS4) or lacking (-AS4) an insertion at splice site 4. Western blot experiments showing the expression of alpha- and beta-Nrxn1 splice isoforms in neuronal cultures transduced with lentiviral vectors specific for neuronal expression

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Molecular Brain

ISSN: 1756-6606