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Fig. 3 | Molecular Brain

Fig. 3

From: Analysis of neurexin-neuroligin complexes supports an isoform-specific role for beta-neurexin-1 dysfunction in a mouse model of autism

Fig. 3

Transcellular recruitment of beta-Nrxn1 at presynaptic contacts mediated by Nlgn1 and Nlgn2. A. Cortical neurons expressing beta-Nrxn1 (-AS4) or beta-Nrxn1 (AS4) were co-cultured with HEK293T cells expressing GFP, Nlgn1 or Nlgn2, as shown. The expression of beta-Nrxn1 splice isoforms and Nlgn1/2 was detected with Flag and VSV antibodies, respectively. B. Graphs showing the quantification of neuronal beta-Nrxn1 splice isoforms recruited at presynaptic sites induced by Nlgn1 and Nlgn2. Note that beta-Nrxn1 proteins are similarly recruited by Nlgn1 and Nlgn2 and that the insertion of AS4 in beta-Nrxn1 decreases presynaptic recruitment. Scale bar, 5 μm for Nlgn1 and Nlgn2 and 6,85 μm for GFP transfections

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Molecular Brain

ISSN: 1756-6606