Fig. 3
Effects of optogenetic inhibition of MD-ACC pathway on shock-responding activity in observer’s ACC. (a) Representative data from Cell #36(B2). Top panel) Mean of GCaMP6f fluorescent activity traces over light-OFF condition (left) and light-ON (right) condition. Yellow color bar indicates the shock period (2 second). Red color bars indicate the red-LED ON period. Middle panel) Raster plots of calcium transients before and after the shock moment over 10 shocks during red light-OFF (left) and light-ON (right) condition. Black line plots indicate the event timing of calcium transients. Yellow color bar indicates the shock period (2 second). Red color bars indicate the red-LED ON period. Bottom panels) Frequency distribution of mean spike rate determined by 1000 times shuffle. Dashed line indicates 95th percentile of the frequency distribution. Blue line indicates mean spike rate observed during shock moment during red light-OFF and light-ON condition. (b) Percentages of 27 SRCs from 3 mice (Fig. 2c) during MD terminal inhibition in ACC (96.4%, p < 0.0001, X2 = 23.148, df = 1)