Fig. 4

Normal fear acquisition and retrieval but impaired fear extinction in mice manipulated with proBDNF-EPE. A: Illustrative scheme of contextual fear conditioning displaying viral injections sites, timeline of the experiment and fear extinction protocol used. B: Mice manipulated with proBDNF-EPE, proNGF-EPE, or GFP control LVs exhibit comparable and normal FC learning curve. C: Mice injected with the proBDNF-EPE LV display higher freezing rates compared to the GFP control and proNGF-EPE experimental groups as of the third day of extinction trial. All data presented as Mean ± SEM. Mixed effects analysis (n ≥ 12, Extinction Day 1: GFP control Versus BDNF-EPE: p = 0.0003, NGF-EPE Versus BDNF-EPE: p = 0.0002. Extinction Day 2: GFP control Versus BDNF-EPE: p < 0.0001, NGF-EPE Versus BDNF-EPE: p = 0.0006. Extinction Day 3: GFP controls Versus BDNF-EPE: p < 0.0001 NGF-EPE Versus BDNF-EPE: p < 0.0001. Extinction Day 4: GFP control Versus BDNF-EPE: p = 0.0012, NGF-EPE Versus BDNF-EPE: p = 0.0015. Extinction Day 5. GFP control Versus BDNF-EPE: p < 0.0001, NGF-EPE Versus BDNF-EPE: p = 0.0056)