Fig. 5

proBDNF-EPE manipulation inhibits ERK nuclear functions and induces GluR2 expression. Hippocampal CA1 regions from both hemispheres of the experimental groups were processed for western blot analysis. The upper panel represents quantified results, and representative immunoblots are displayed in the lower panel. A, B: Phospho-ERK1/2 levels are presented as the ratio between phospho-ERK1/2 and total ERK1/2. Results were normalized to control group (n = 8, ERK1: GFP Control versus proBDNF-EPE, p = 0.0010, ERK2: GFP Control versus proBDNF-EPE, p = 0.0273). C: Phospho-Elk1 levels are presented as the ratio between phospho-Elk1 and total Elk1. Results were normalized to GFP control group (n ≥ 8, GFP Control versus proBDNF-EPE, p = 0.0261). D: Phospho-MSK1 levels are presented as the ratio between phospho-MSK1 and total MSK1 (n ≥ 8, GFP Control versus proBDNF-EPE, p = 0.0394) E: GluR2 levels are presented as the ratio between GluR2 and β-Actin. Results were normalized to GFP control group (n ≥ 8, GFP Control versus proBDNF-EPE, p = 0.0131). All data are presented as mean ± SEM