Fig. 7

Inhibition of ERK nuclear translocation increases the frequency of mEPSCs in CA1 pyramidal neurons. A: The experimental design and whole-cell patch clamp recordings made from CA1 pyramidal neurons expressing GFP/proBDNF-EPE. Arrowhead showing the recording pipette. Scale 10 μm. B: Representative traces of mEPSCs recordings from controls (Green, an average of n = 8 similar traces) and peptide (blue, an average of n = 9 similar traces), from 4 animals each. Scale bars 20 mV and 10 s. C: Cumulative probability distributions and dot plots of mEPSCs inter-event intervals. Increased mEPSCs frequencies in proBDNF-EPE-treated animals (0.7167 ± 0.1111 Hz) compared to the controls (0.3771 ± 0.05554 Hz), p = 0.0190, Unpaired t-test. D: Cumulative probability distributions and dot plots of mEPSCs amplitudes. There was no significant difference in the amplitude of mEPSCs in comparing proBFND-EPE treated animals (15.92 ± 1.438 pA) and controls (13.73 ± 0.9057 pA), p = 0.2359, Mann Whitney test. Mann-Whitney U = 23