Fig. 1

Changes in gene expression in the visual cortex under the influence of psilocybin and/or visual sensory excitation. A.) Schematic representation of experimental design, created with BioRender.com. B.) Principal Component Analysis (PCA) representing gene expression in all experimental groups (n = 6). C.) volcano plot representing gene expression changes in the light-induced genes (PBS Dark vs. PBS Light) and psilocybin-induced genes (PBS Dark vs. Psi Dark) treatment. D.) Venn diagram representing overlapping changes in gene expression of light-induced genes and psilocybin-induced genes (adjusted q-value < 0.05). E.) Graphs of representative genes in which gene expression significantly changed due to both psilocybin and light exposure separately. Values represent mean ± SEM, n = 6, ∗/∗∗/∗∗∗FDR < 0.05/0.01/0.001 (Deseq2 Wald test with correction for multiple tests). F.) Validation experiment of RNA-seq analysis by Quantitative PCR analysis on representative genes in the visual cortex in which gene expression significantly changed due to both psilocybin and light exposure separately. Values represent mean ± SEM, n = 6, ∗/∗∗/∗∗∗p < 0.05/0.01/0.001 by one-way ANOVA, followed by Tukey’s multiple comparisons test. G.) Gene Ontology (GO) enrichment analysis for genes regulated by both light and psilocybin. Gene ontology categories with FDR < 0.05 are shown. H.) Heatmap representing genes significantly changed in mRNA expression due to synergistic effect of psilocybin and light. I.) Gene Ontology (GO) enrichment analysis for genes regulated by psilocybin-light additive effect. Gene ontology categories with FDR < 0.05 are shown. J.) Graphs represent genes in which mRNA expression significantly changed due to the synergistic effect of psilocybin and light exposure. Values represent mean ± SEM, n = 6, ∗/∗∗/∗∗∗FDR < 0.05/0.01/0.001 (Deseq2 Wald test with correction for multiple tests).