Fig. 2
From: Comparative experience shapes sucrose preference through memory in Drosophila
Previous experience enhances preference for 150 mM sucrose concentration on low-well configurations. (A, C, E, G, I, K, M) Schematic representations of the two-choice feeding assay for naive flies (top) and experienced flies (bottom). Naive flies were tested on a plate with a 4:4 configuration (test plate) for 15 min, while experienced flies underwent a pre-exposure period in a 30:30 configuration plate for 3 min followed by testing on a test plate unless otherwise stated. (B) PI for 150 mM solution on the test plate. Naive flies showed a median PI of 29.58% while experienced flies a 42.08%. (C) Representation of the feeding assay switching the red and blue dye. (D) Naive flies’ PI 9.59%, experienced flies’ PI 33.02%. (E) Representation of the feeding assay with a 2:2 configuration test plate. (F) Naive flies’ PI 17.86%, experienced flies’ PI 49.26% (G) Representation of the feeding assay with a 4:4 asymmetrical configuration test plate. (H) Naive flies’ PI 29.70%, experienced flies’ PI 60.08% (I) Representation of the feeding assay with a 4:4 configuration pre-exposure plate. (J) Naive flies’ PI 25.6%, experienced flies’ PI 25.15% (K) Representation of the feeding assay with a 4:4 configuration pre-exposure plate and a 30:30 configuration test plate. (L) Naive flies’ PI 72.31%, experienced flies’ PI 77.66% (M) Representation of the feeding assay with a 30-, 60-, and 120-minutes wait between the pre-exposure and the test. (N) Naive flies’ PI 25.14%, 30 min wait flies’ PI 41.88%, 60 min wait flies’ PI 40.41%, 120 min wait flies’ PI 41.97%. Each circle represents the preference index using about 40 flies. Box plots show median, 25th, and 75th percentiles and the whisker bars represent maximum and minimum values. Statistical significance was determined using a two-tailed Student’s T-test in B, D, F, H, J and L. Statistical significance in N was determined using a one-way ANOVA followed by a Dunnett post hoc testing