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Fig. 2 | Molecular Brain

Fig. 2

From: Development of Cre-dependent retrograde trans-multisynaptic tracer based on pseudorabies virus bartha strain

Fig. 2

The characteristic of PRV676 in vitro. (A) The blue fluorescent protein (BFP) was expressed when the PRV676 infected the BHK-21 cells, which provided a direct signal for virus production in vitro. (B) The PRV676 can infect BHK-21 cells and produce plaque. (C) The growth curve of PRV676. The BHK-21 cells were infected by the PRV676 at an MOI of 0.1. The supernatant was collected at 12, 24, 36, 48, 60, and 72 hpi, and the titers of these samples were determined by using plaque assay. (D) The EGFP was expressed under the control of Cre. (E) The time course of EGFP expression and virus production in BHK-21 cells expressing Cre. The EGFP signals were observed at 12, 24, 36, 48, 60, and 72 hpi

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Molecular Brain

ISSN: 1756-6606