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Fig. 3 | Molecular Brain

Fig. 3

From: Development of Cre-dependent retrograde trans-multisynaptic tracer based on pseudorabies virus bartha strain

Fig. 3

The characteristic of PRV829 in vitro. (A) The blue fluorescent protein (BFP) was expressed when the PRV829 infected the BHK-21 cells, which provided a direct signal for virus production in vitro. (B) The PRV829 can infect BHK-21 cells and produce plaque. (C) The growth curve of PRV829. The BHK-21 cells were infected by the PRV829 at an MOI of 0.1. The supernatant was collected at 12, 24, 36, 48, 60, and 72 hpi, and the titers of these samples were determined by using plaque assay. (D) The mRuby3 was expressed under the control of Cre. (E) The time course of mRuby3 expression and virus production in BHK-21 cells expressing Cre. The mRuby3 signals were observed at 12, 24, 36, 48, 60, and 72 hpi

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Molecular Brain

ISSN: 1756-6606