Fig. 1
Effect of Hcy on the electrical excitability of cultured hippocampal neurons. a Representative recordings of AP series evoked by a 300-ms depolarizing current pulse (300 pA) under control conditions, after acute application of 300 µM Hcy, and after chronic application of 100 µM Hcy, as indicated. Insets show the first AP in each series on an expanded time scale. b Representative 160-ms segments extracted from 5-minute recordings of spontaneous neuronal activity under the same conditions as in panel (a). c Number of APs per series recorded as in panel (a) during acute (top panel) and chronic (bottom panel) Hcy application at the indicated concentrations. *p = 0.0119. d Rise time of the first AP in each series recorded as in panel (a) during acute (top panel) and chronic (bottom panel) Hcy application at indicated concentrations. Numbers within the bars represent the number of neurons analyzed. *p = 0.0176; ***p = 0.0003. e Cumulative histograms of interspike intervals (ISI) distributions during 5-minute recordings of spontaneous neuronal activity under control conditions and in the presence of Hcy, as indicated. All experiments were performed on neuronal cultures derived from five independent litters