Fig. 5
From: GAS reduced inflammatory responses in activated microglia by regulating the Ccr2/Akt/Gsk-3β pathway
GAS activates the Ccr2/Akt/Gsk-3β pathway in OGD-induced BV2 cells. A-E Fluorescence images showed that compared with the control group, the levels of Ccr2 (red) in Lectin + microglia (green) were significantly increased, while the levels of p-Akt (red) and p-Gsk-3β (red) were significantly decreased in the OGD group. After GAS treatment, the expression of Ccr2 decreased, and the levels of p-Akt and p-Gsk-3β increased. DAPI (blue) indicates the nucleus. F Presents the average immunofluorescence values of Ccr2, Akt, p-Akt, Gsk-3β, and p-Gsk-3β. n = 3, *P < 0.05; **P < 0.01, ***P < 0.001. Scale: 50 μm